Cleto rodriguez biography samples

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    Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii

    • Research
    • Open access
    • Published:

    Microbial Cell Factoriesvolume 23, Article number: 263 (2024) Cite this article

    Abstract

    Background

    The β-galactosidase from Paenibacillus wynnii (β-gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low KM value) compared to industrially used β-galactosidases and is not inhibited by the hydrolysis-generated product D-galactose. However, β-gal-Pw must firstly be produced cost-effectively for any potential industrial application. Accordingly, the yeast Komagataella phaffii was chosen to investigate its feasibility to recombinantly produce β-gal-Pw since it is approved for the regulated production of food enzymes. The aim of this study was to find the most suitable way to produce the β-gal-Pw in K. phaffii either extracellularly or in

  • cleto rodriguez biography samples

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    ABSTRACT

    Lytic bacteriophages are expected as effective tools to control infectious bacteria in human and pathogenic or spoilage bacteria in foods. Leaderless bacteriocins (LLBs) are simple bacteriocins produced by Gram-positive bacteria. LLBs do not possess an N-terminal leader peptide in the precursor, which means that they are active immediately after translation. In this study, we constructed a novel antimicrobial agent, an LLB-producing phage (LLB-phage), by genetic engineering to introduce the LLB structural gene into the lytic phage genome. To this end, lnqQ (structure gene of an LLB, lacticin Q) and trxA, an essential gene for T7 phage genome replication, were integrated in tandem into T7 phage genome using homologous recombination in Escherichia coli host strain. The recombinant lnqQ-T7 phage was isolated by a screening method using ΔtrxA host strain. lnqQ-T7 phage formed a clear halo in agar plates containing both E. coli and lacticin Q-susceptible Bacillus

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    Floyd Mayweather Jr. vs. Manny Pacquiao

    2015 professional boxing match

    DateMay 2, 2015
    VenueMGM Grand Garden Arena, Paradise, Nevada, U.S.
    Title(s) on the lineWBA (Unified), WBC, WBO, and The Ring welterweight titles
    BoxerFloyd Mayweather Jr.Manny Pacquiao
    Nickname Money Pac-Man
    HometownGrand Rapids, Michigan, U.S.General Santos, Philippines
    Pre-fight record 47–0 (26 KO) 57–5–2 (38 KO)
    Age 38 years, 2 months 36 years, 4 months
    Height 5 ft 8 in (173 cm) 5 ft 5+1⁄2 in (166 cm)
    Weight 146 lb (66 kg) 145 lb (66 kg)
    StyleOrthodoxSouthpaw
    RecognitionWBA (Unified), WBC, and The Ring welterweight champion
    WBA (Super), WBC, and The Ring light middleweight champion
    The Ring No. 1 ranked pound-for-pound fighter
    5-division world champion    		WBO welterweight champion
    The Ring No. 3 ranked pound-for-pound fighter
    8-division w